Procoagulant activity of platelet arachidonic acid metabolites

Enhancement of mononuclear procoagulant activity by platelet 12-hydroxyeicosatetraenoic acid.

Platelets induce generation of procoagulant tissue factor activity (TFa) by mononuclear leukocytes, and also enhance the TFa induced by endotoxin. Our present investigation demonstrated that arachidonic acid, which by itself had no effect on mononuclear TFa, greatly enhanced platelet-induced TFa. The effect was concentration dependent for both platelets and arachidonate (1-20 microM); other fat...

Arachidonic Acid Metabolites in Inflammation

Cyclosporine enhances platelet procoagulant activity.

BACKGROUND Clinical use of cyclosporine (CsA) was suggested to be associated with an increased risk of thromboembolic complications. The molecular mechanisms underlying these effects remain unresolved. METHODS We tested the hypothesis that CsA may produce platelet procoagulant activity due to its interaction with the platelet plasma membrane. To verify this hypothesis the possible relationshi...

Regulation of arachidonic acid metabolites in macrophages

The lipids of mouse peritoneal macrophages contain high levels (25 mole percent) of esterified arachidonic acid (20:4). Following in vitro exposure to unopsonized zymosan, these cells synthesize and release oxygenated products of 20:4. Maximal levels of zymosan ingestion promote the release of 40-50% of the 20:4 content of cultures without loss of viabilitiy. Release of radiolabel from macropha...

Lipoxygenase metabolites of arachidonic acid modulate hematopoiesis.

Lipoxygenase (LPO) metabolites of arachidonic acid participate in the activation and/or proliferation of a variety of cell types. In this study, we examined the role of LPO metabolites in controlling myelopoiesis and erythropoiesis in vitro. Monocyte depleted cells (MDC) prepared from human whole blood or whole mononuclear cells from human bone marrow were cultured in methylcellulose in the pre...